CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement
نویسندگان
چکیده
منابع مشابه
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Recent developments in genome editing techniques have aroused substantial excitement among agricultural scientists. These techniques offer new opportunities for developing improved plant lines with addition of important traits or removal of undesirable traits. Increased adoption of genome editing has been geared by swiftly developing Clustered regularly interspaced short palindromic repeats (CR...
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Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of recombinant Cpf1 proteins with in vitro transcribed or chemically synthesized target-specific cr...
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Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) has been setting the world of genome editing ablaze due to its ease of application and the precise nature of its targeting. Even though Cas9 has emerged as the most commonly used CRISPR effector for genome editing-based experiments, certain limitations in its targeting location (targets only GC rich area) and the creation of blu...
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The biotechnology sector is rapidly changing with the increase in technological advancements. 1 The laws governing patent protection, specifically the laws governing patent eligibility, have also changed to adapt to these innovations.2 This paper focuses on the CRISPR-Cas9 technology, a genome editing tool that is changing the field of genetic engineering.3 As of November 2016, the U.S. Patent ...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2020
ISSN: 1664-462X
DOI: 10.3389/fpls.2020.584151